Time-resolved nanocrystallography with X-ray lasers

Time-resolved protein nanocrystallography using an X-ray free-electron laser

We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredoxin co-crystals were observed at time delays of 5 to 10 µs after excitation. The result correlates...

Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers

Knowledge of protein structure provides essential insight into function, enhancing our understanding of diseases and enabling new treatment development. X-ray crystallography has been used to solve the structures of more than 100 000 proteins; however, the vast majority represent long-lived states that do not capture the functional motions of these molecular machines. Reactions triggered by the...

Optimum driving a Z-pinch for soft X-Ray lasers

A capillary plasma z-pinch as an alternative active medium of soft X-Ray lasers was studied experimentally and theoretically. The theoretical analysis was based on the self consistent solution of the so called “snow plow” model. The dynamics of pinched plasma is determined by the capillary parameters and by the time dependence of electrical current passing through it. The current time dependenc...

Time Resolved Spectroscopy with Femtosecond X-Ray Pulses

Time resolved photoelectron spectroscopy with ultrafast soft x-ray light

2001 This thesis entitled: Time resolved photoelectron spectroscopy with ultrafast soft x-ray light written by Lora Nugent-Glandorf has been approved for the Department of Chemistry and Biochemistry Date The final copy of this thesis has been examined by the signatories, and we find that both the content and the form meet acceptable presentation standards of scholarly work in the above mentione...